Nanobiotechnology


Erman A, Kapun G, Novak S, Pavlin M, Dražič G, Drobne D, Veranič P. eter. How cancer cells attach to urinary bladder epithelium in vivo : study of the early stages of tumorigenesis in an orthotopic mouse bladder tumor model. Histochemistry and cell biology, ISSN 0948-6143, Mar. 2019, vol. 151, iss. 3, str. 263-273  https://link.springer.com/content/pdf/10.1007%2Fs00418-018-1738-x.pdf

Lojk J, Bregar VB, Strojan K, Hudoklin S, Veranič P, Pavlin M, Erdani-Kreft M. Increased endocytosis of magnetic nanoparticles into cancerous urothelial cells versus normal urothelial cells. Histochemistry and cell biology, 2018;149(1):45-59 DOI: https://doi.org/10.1007/s00418-017-1605-1,  https://link.springer.com/article/10.1007%2Fs00418-017-1605-1

Lojk J, Strojan K, Miš K, Bregar VB, Hafner Bratkovič I, Bizjak M, Pirkmajer S, Pavlin M. Cell stress response to two different types of polymer coated cobalt ferrite nanoparticles. Toxicology letters, 2017, vol. 270, str. 108-118 http://www.sciencedirect.com/science/article/pii/S0378427417300589

Strojan K, Lojk J, Bregar VB, Erdani-Kreft M, Svete J, Veranič P, Pavlin M. In vitro assessment of potential bladder papillary neoplasm treatment with functionalized polyethyleneimine coated magnetic nanoparticles. Acta chimica slovenica, 2017, 64(3):543-548  https://journals.matheo.si/index.php/ACSi/article/view/2876/1249

Lojk J, Prpar Mihevc S, Bregar VB, Pavlin M, Rogelj B. The effect of different types of nanoparticles on FUS and TDP-43 solubility and subcellular localization. Neurotoxicity research, 2017, vol. , 1-15, http://link.springer.com/article/10.1007/s12640-017-9734-9

Strojan K, Lojk J, Bregar VB, Veranič P, Pavlin M. Glutathione reduces cytotoxicity of polyethyleneimine coated magnetic nanoparticles in CHO cells. Toxicology in vitro, 2017, vol. 41, str. 12-20, http://www.sciencedirect.com/science/article/pii/S0887233317300292

Strojan K, Leonardi A, Bregar VB, Križaj I, Svete J, Pavlin M. Dispersion of Nanoparticles in Different Media Importantly Determines the Composition of Their Protein Corona, PLOS ONE, 2017, 12(1): e0169552.  http://dx.doi.org/10.1371/journal.pone.0169552

Lojk J, Čibej U, Karlaš D, Šajn L, Pavlin M. Comparison of two automatic cell-counting solutions for fluorescent microscopic images. Journal of Microscopy, 2015, vol. 260, str. 107-116, http://onlinelibrary.wiley.com/doi/10.1111/jmi.12272/epdf

Lojk J, Bregar VB, Rajh M, Miš K, Erdani-Kreft M, Pirkmajer S, Veranič P, Pavlin M. Cell type-specific response to high intracellular loading of polyacrylic acid-coated magnetic nanoparticles. International journal of nanomedicine, 2015, vol. 10, str. 1449-1462 http://www.dovepress.com/cell-type-specific-response-to-high-intracellular-loading-of-polyacryl-peer-reviewed-article-IJN

Bregar VB, Lojk J, Šuštar V, Veranič P, Pavlin M. Visualization of internalization of functionalized cobalt ferrite nanoparticles and their intracellular fate. International journal of nanomedicine, 2013, vol. 8, str. 919-931 http://www.dovepress.com/visualization-of-internalization-of-functionalized-cobalt-ferrite-nano-peer-reviewed-article-IJN

Pavlin M, Bregar VB. Stability of nanoparticle suspensions in different biologically relevant media. Digest Journal of Nanomaterials and Biostructures, 2012, vol. 7, 1389-1400 http://www.chalcogen.infim.ro/1389_Bregar.pdf

 

 

Metabolism of cancer cells


Bizjak M, Malavašič P, Pirkmajer S, Pavin M. Comparison of the effects of metformin on MDA-MB-231 breast cancer cells in a monolayer culture and in tumor spheroids as a function of nutrient concentrations. Biochemical and biophysical research communications, doi: 10.1016/j.bbrc.2019.05.090,  https://www.sciencedirect.com/science/article/pii/S0006291X19309660?via%3Dihub

Dolinar K, Jan V, Pavlin M, Chibalin A, Pirkmajer S. Nucleosides block AICAR-stimulated activation of AMPK in skeletal muscle and cancer cells. American journal of physiology, Cell physiology 2018, doi: 10.1152/ajpcell.00311.2017https://www.physiology.org/doi/abs/10.1152/ajpcell.00311.2017

Bizjak M, Malavašič P, Dolinar K, Pohar J, Pirkmajer S, Pavlin M. Combined treatment with Metformin and 2-deoxy glucose induces detachment of viable MDA-MB-231 breast cancer cells in vitro. Scientific Reports 7:1761, 2017 http://www.nature.com/articles/s41598-017-01801-5

Rajh M, Dolinar K, Miš K, Pavlin M, Pirkmajer S. Medium renewal blocks anti-proliferative effects of metformin in cultured MDA-MB-231 breast cancer cells. PloS one, May 2, 2016, str. 1-18 doi: https://doi.org/10.1371/journal.pone.0154747  http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154747

 

Gene electrotransfer and cell electroporation


Pavlin M and Kandušer M. New insights into the mechanisms of gene electrotransfer – experimental and theoretical analysis. Scientific reports, 2015, 5: 1-11 http://www.nature.com/srep/2015/150316/srep09132/full/srep09132.html

Lojk J, Miš K, Pirkmajer S, Pavlin M. siRNA delivery into cultured primary human myoblasts – optimization of electroporation parameters and theoretical analysis. Bioelectromagnetics, 2015, 36: 551-563 http://onlinelibrary.wiley.com/doi/10.1002/bem.21936/abstract

Marš T, Stražišar M, Miš K, Katarina P, Kotnik N, Pegan K, Lojk J, Grubič  Z, Pavlin M. Electrotransfection and lipofection show comparable efficiency for In vitro gene delivery of primary human myoblasts. The journal of membrane biology, 2015, 248: 273-283 https://link.springer.com/article/10.1007%2Fs00232-014-9766-5

Haber Meglič  S, Kandušer M, Flisar K, Hodžić D, Bregar VB, Miklavčič D, Escoffre JM, Rols MP, Pavlin M. Effect of different parameters used for in vitro gene electrotransfer on gene expression efficiency, cell viability and visualization of plasmid DNA at the membrane level. The journal of gene medicine, 2013, 15: 169-181 http://onlinelibrary.wiley.com/doi/10.1002/jgm.2706/pdf

Pavlin M, Pucihar G, Kandušer M. The role of electrically stimulated endocytosis in gene electrotransfer. Bioelectrochemistry, 2012, 83: 38-44 https://link.springer.com/chapter/10.1007/978-3-642-13039-7_171

Faurie C, Reberšek M, Golzio M, Kandušer M, Escoffre JM, Pavlin M, Teissié J, Miklavčič D, Rols MP. Electro-mediated gene transfer and expression are controlled by the life-time of DNA/membrane complex formation. J. Gene Med. 12: 117-125, 2010.  http://onlinelibrary.wiley.com/doi/10.1002/jgm.1414/abstract

Haberl S, Pavlin M. Use of collagen gel as a three-dimensional in vitro model to study electropermeabilization and gene electrotransfer. J. Membrane Biol. 236: 87-95, 2010.  https://link.springer.com/article/10.1007/s00232-010-9280-3

Marjanovič I, Haberl S, Miklavčič D, Kandušer M, Pavlin M. Analysis and comparison of electrical pulse parameters for gene electrotransfer of two different cell lines. J. Membrane Biol. 236: 97-105, 2010. https://link.springer.com/article/10.1007%2Fs00232-010-9282-1

Haberl S, Miklavčič D, Pavlin M. Effect of Mg ions on efficiency of gene electrotransfer and on cell electropermeabilization. Bioelectrochemistry 79: 265-271, 2010. http://www.sciencedirect.com/science/article/pii/S156753941000068X

Pavlin M, Flisar K, Kandušer M. The role of electrophoresis in gene electrotransfer. J. Membrane Biol. 236: 75-79, 2010.  https://link.springer.com/article/10.1007/s00232-010-9276-z

Županič A, Čorović S, Miklavčič D, Pavlin M. Numerical optimization of gene electrotransfer into muscle tissue. Biomed. Eng. Online 9: 66, 2010. http://www.biomedical-engineering-online.com/content/9/1/66

Kandušer M, Miklavčič D, Pavlin M. Mechanisms involved in gene electrotransfer using high- and low-voltage pulses – an in vitro study. Bioelectrochem. 74: 265-271, 2009. http://www.sciencedirect.com/science/article/pii/S1567539408001515

Pavlin M, Miklavčič D. Theoretical and experimental analysis of conductivity, ion diffusion and molecular transport during cell electroporation – relation between short-lived and long-lived pores. Bioelectrochem. 74: 38-46, 2008.  http://www.sciencedirect.com/science/article/pii/S1567539408000698?via%3Dihub

Čorović S, Pavlin M, Miklavčič D. Analytical and numerical quantification and comparison of the local electric field in the tissue for different electrode configurations. Biomed. Eng. Online 6 : 1-14, 2007.

Pavlin M, Kandušer M, Reberšek M, Pucihar G, Hart FX, Magjarević R, Miklavčič D. Effect of cell electroporation on the conductivity of a cell suspension. Biophys. J. 88: 4378-4390, 2005.

Valič B, Pavlin M, Miklavčič D. The effect of resting transmembrane voltage on cell electropermeabilization: a numerical analysis. Bioelectrochemistry 63: 311-315, 2004.

Valič B, Golzio M, Pavlin M, Schatz A, Faurie C, Gabriel B, Teissié J, Rols MP, Miklavčič D. Effect of electric field induced transmembrane potential on spheroidal cells: theory and experiment. Eur. Biophys. J. 32: 519-528, 2003.

Pavlin M, Miklavčič D. Effective conductivity of a suspension of permeabilized cells: a theoretical analysis. Biophys. J. 85: 719-729, 2003

Pavlin M, Pavselj N, Miklavčič D. Dependence of induced transmembrane potential on cell density, arrangement, and cell position inside a cell system. IEEE Trans. Biomed. Eng. 49: 605-612, 2002.

Pavlin M, Slivnik T, Miklavčič D. Effective conductivity of cell suspensions. IEEE T. Biomed. Eng. 49: 77-80, 2002.

 

Numerical modelling for industrial applications


Strauch L, Pavlin M, BREGAR, Bregar VB. Optimization, design, and modeling of ferrite core geometry for inductive wireless power transfer. International journal of applied electromagnetics and mechanics, ISSN 1383-5416, 2015, vol. 49: 145-155,  doi: 10.3233/JAE-150029.

Drnovšek B, Bregar VB, Pavlin M. The effect of complex permeability and agglomeration on composite magnetic systems : a three-dimensional numerical analysis and comparison with analytical models. Journal of applied physics, 2008, vol. 103, no. 7: 07D9241/1-3

Drnovšek B, Bregar VB, Pavlin M. Numerical study of effective permeability of soft-magnetic composites with conductive inclusions. J. Appl. Phys. 105: 07D546 , 2009

 

 

Book chapters


Pavlin M, Zupančič D, Lojk J, Strojan K, Erdani-Kreft M. Multimodal magnetic nanoparticles for biomedical applications: importance of characterization on biomimetic in vitro models. Materials for biomedical engineering : inorganic micro- and nanostructures. 1st ed. Amsterdam; Oxford; Cambridge: Elsevier. 2019, p. 241-283,  https://www.sciencedirect.com/science/article/pii/B9780081028148000093

Pavlin M and Kandušer M. Gene electrotransfer : from understanding the mechanisms to optimization of parameters in tissues. V: IGLIČ, Aleš (ur.). Advances in planar lipid bilayers and liposomes, (Advances in planar lipid bilayers and liposomes, ISSN 1554-4516, vol. 15). Amsterdam [etc.]: Elsevier: Academic Press, 2012, str. 77-104, http://dx.doi.org/10.1016/B978-0-12-396533-2.00001-X.

Pavlin M, Kotnik T, Miklavčič D, Kramar P, Maćek-Lebar A. Electroporation of planar lipid bilayers and membranes. V: OTTOVA-LEITMANNOVA, Angelica (ur.). Advances in planar lipid bilayers and liposomes. Vol. 6. Amsterdam [etc.]: Academic Press, Elsevier Science, cop. 2008, str. 165-226