Our facilities consist of i) fully equipped laboratory for synthesis of nanoparticles and ii) fully equipped cell culture laboratory.
Methods available in our laboratories:
- Synthesis of magnetic and gold nanoparticles,
- Culturing of numerous mammalian cell lines and cell models,
- Viability and proliferation assays (MTS, Propidium iodide, trypan blue exclusion, BrdU),
- Reactive oxygen species measurements,
- High-voltage electroporation and electrotransfection enabling various protocols for electroporation of cells in vitro and in vivo for RNAi/DNA/small molecules electrotransfer,
- Western blot,
- Fluorescence microscopy,
- Endotoxin analysis and,
- Flow cytometry.
Methods available to us trough collaboration:
- Dynamic light scattering and zeta potential measurements,
- Transmission and scanning electron microscopy (TEM, SEM),
- SDS polyacrylamide gel electrophoresis (SDS-PAGE),
- Two dimensional gel electrophoresis (2-DE),
- Mass spectrometry (MS),
- Enzyme-linked immunosorbent assay (ELISA),
- Quantitative polymerase chain reaction (qPCR),
- Flow cytometry.